Its putative promoter region was highly homologous to B-dependent promoter of B. subtilis. A 2.6 kb fragment including the betH gene was subcloned into pUC18 and transformed into the E.
英
美
- 將包括整個(gè)betH基因ORF框及可能的啟動(dòng)子核苷酸序列在內的2.；6kb的片段克隆到pUC18載體上，轉入到大腸桿菌甘氨酸甜菜堿缺失株MKH13中，使該菌株能夠在含甘氨酸甜菜堿的高鹽M9培養基上生長(cháng)，而對照實(shí)驗不能生長(cháng)。
