In this experiment, differential expressing genes in leg muscle of chicken mentioned above were studied via DDRT-PCR technique, which firstly adopted 72 primer pairs that piloted by 3 T7 (dT11) N as anchor primers and 24 M13-ARPs as random primers.
英
美
- 本實(shí)驗采用3個(gè)26bp的T7(dT11)N為錨定引物,24個(gè)26bp的M13-ARPs為隨機引物,組成72個(gè)引物組合對上述四個(gè)品種雛雞腿肌組織進(jìn)行DDRT-PCR反應。