In order to establish a specific detecting method to AEV, monoclonal antibody against AEV was prepared with AEV purified by cesium-chloride equilibrium centrifugation propagated using SPF eggs via the yolk sac inoculation.
英
美
- 國外已有用AEV單克隆抗體(VR9-1株)建立免疫組化、間接免疫熒光和免疫過(guò)氧化物酶染色、抗生物素蛋白-生物素-過(guò)氧化物酶染色方法檢測AEV抗原的成功報道,并用于A(yíng)EV的定量研究,而國內相關(guān)報道較少,成功應用的更少。
