Immunofluorescence analysis. Primary antibodies were monoclonal antibodies of p21, cyclin D1, CDK4. FITC-labeled secondary antibodies were used. The fluorescence was measured by Coulter EPICS XL.

 
  • 免疫熒光分析:第一抗體為細胞周期蛋白D1(cyelin D1)、細胞周期蛋白依賴(lài)性激酶4(CDK4)、p21蛋白的單克隆抗體,第二抗體采用熒光素標記,應用流式細胞儀檢測熒光強度。
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