ImmunocytochemistryThe BME - induced mMSCs were fixed with 4% paraformalde-hyde, incubated with primary antibodies of NSE and GFAP overnight at 4C , incubated with secondary antibody for 30min.
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- brillny acidic protein,GFAP)的表達情況。 2.;3 免疫細胞化學(xué)檢測誘導后細胞NSE和GFAP的表達將用BME誘導后的細胞爬片用4%25多聚甲醛固定30分鐘,·2·0