Hydrophobic area was detected to be present,in TKL by the interaction with fluorescene probe TNS,and this hydrophobic area was different from the galactose binding site of TKL. There was no evidence to show metal-binding site in TKL moleclule.

 
  • 疏水探針TNS能夠檢測到TKL中疏水微區的存在,并且這一疏水微區亦不同于TKL的半乳糖結合位點(diǎn),TKL中不存在金屬離子的結合部位。
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