Human ScFv against botulinum neurotoxin serotype A(BoNTa) was modified by fusing human IgG1 Fc to C terminal of ScFv. ScFv-Fc fusion protein was expressed at high level over 30% of total host cell proteins in E. coli.
英
美
- 以獲得的抗A型肉毒毒素單鏈抗體為模板,進(jìn)行融合改構,將人IgG1的Fc片段連接到ScFv的C端,在大腸桿菌中實(shí)現抗體融合蛋白ScFv-Fc的表達,表達量30%25以上,蛋白以包含體形式存在,經(jīng)過(guò)體外變復性的抗體融合蛋白ScFv-Fc,進(jìn)行Prote in G Sepharose柱親和層析純化,純度達90%25~95%25。
