However, the MPH is reversibly inactivated by diethyl pyrocarbonate (DEPC) and the inactivation rate is 9.6 h~(-1). When adding neutralized hydroxylamine, the enzymatic activity of inactivated-MPH was restored to about 60% of initial activity.

 
  • 然而二乙基焦磷酸酯對組氨酸側鏈的化學(xué)修飾引起酶活性的大幅度的下降,其對酶活性的抑制率達到9.;6h~(-1),當在酶的抑制體系中加了羥胺后,MPH的酶活力能夠恢復到原來(lái)的60%25。
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