HSF and HSC-T6 in logarithmic growth phase were incubated in culture plates in which the cultured cell density were adjusted to 1 x lOVml. OD values of the cells were tested by the method of MTT after 24hr incubation.
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- 取對數生長(cháng)期培養細胞HSF,HSC一T6,調整細胞密度為lxl05個(gè)/m1,接種于%25孔板24小時(shí)后,分別加入不同濃度pHGF,孵育24小時(shí),進(jìn)行MTT試驗,檢測55Onm0D值。