Guanosine overproducing mutant was derived from Bacillus subtilis T1001 by using ultraviolet and diethyl sulfate (DES). The mutant was selected directionally and step wisely on the agar plates containing methionine sulfoxide(MSO) and 8 azaguanine(8 AG).
英
美
- 以枯草芽孢桿菌T1 0 0 1為出發(fā)菌株 ,經(jīng)紫外線(xiàn)、硫酸二乙酯逐級誘變處理 ,選育出腺嘌呤缺陷型 (Ade- )菌株 ,然后經(jīng)蛋氨酸亞砜 (MSO)、8 氮鳥(niǎo)嘌呤 ( 8 AG)結構類(lèi)似物平板定向篩選 ,獲得鳥(niǎo)苷高產(chǎn)菌株TA2 0 8。
