G3P DHG was purified 123.6-fold by gel filtration on Sephadex G-25, affinity chromatography on blue Sepharose and ion-exchange chromatography on Mono Q.

 
  • 粗酶液經(jīng)Sephadex G-25凝膠過(guò)濾;Blue Sepharose親和層析以及Mono Q離子交換等步驟;提純123.;6倍;得純酶液。
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