Fipronil(F) and one of its analogy(FH)were adopted as haptens0 The former was coupled to carrier protein by both EDC and GD, and the later was coupled to carrier protein by active ester.

 
  • 本文采用氟蟲(chóng)腈(F)及其一種類(lèi)似物(FH)作為半抗原,分別通過(guò)碳化二亞胺法(EDC)和戊二醛法把氟蟲(chóng)腈偶聯(lián)到載體蛋白上,通過(guò)活性酯法把FH偶聯(lián)到載體蛋白上,作為人工抗原。
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