Derivatization of FLX and NFLX with NBD-COC1 and DBD-COC1.As the fluorescent labeling reagents, NBD-COC1 and DBD-COC1, both of which has an acyl halide group, were selected to derivatize the amines.
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- NBD-COC1的氟西汀和去甲氟西汀衍生物(NBD-FLX和NBD-NFLX)的最大熒光激發(fā)和發(fā)射波長(cháng)分別為478±2nm和537±2nm,DBD-COC1的氟西汀和去甲氟西汀衍生物(DBD-FLX和DBD-NFLX)的最大熒光激發(fā)和發(fā)射波長(cháng)分別為454±2nm和562±2nm。