Densitometric analysis of the gel indicated that this protein represented 10. 5% of total cellular proteins. The fusion protein was cleaved by CNBr, and the yield of biologically active HV3 was about 20 ATU/ml culture.
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- 該質(zhì)粒在大腸桿菌 JM105宿主細胞中表達后，融合蛋白（15.；6 kD）占細菌總蛋白 10 5%25，該融合蛋白經(jīng) CNBr切割釋放出活性水蛭素分子，抗凝血活力達約 20 ATU/ml培養液，為以后進(jìn)一步研究打下了基礎。
