DNA sequencing result showed that the terminative code of nm23-Hl gene in pLXSN-nm23-H1-EGFP has been mutated from ATG to TGA. The fusion protein of nm23-Hl-EGFP is stablely, continuously and high efficiently expressed in transfected L9981 cell line.

 
  • 應用質(zhì)粒DNA的轉化,酶切,連接技術(shù)成功構建了逆轉錄病毒真核表達載體pLXSN一nm23一Hl一EGFP,將質(zhì)粒pLXSN一nm23一HI一EGFP轉染L9981細胞株后,nm23一Hl一EGFP融合蛋白能夠在受染細胞內持續,穩定和高效表達。
今日熱詞
目錄 附錄 查詞歷史
国内精品美女A∨在线播放xuan