DNA sequencing indicated that the inserted fragment in pBluescript-SK-II vector was HNP-3 mature peptide gene sequence,and the sub-cloned recombinant pGBKT7-HNP-3 was no mismatch.

 
  • 構建的重組質(zhì)粒pBluescript-SK-II-HNP-3,經(jīng)測序鑒定,插入片段為HNP-3成熟肽的cDNA序列。 亞克隆構建的重組誘餌質(zhì)pGBKT7-HNP-3,測序鑒定表明其插入目的基因序列無(wú)誤。
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