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- Bioactivity and safety of direct intramyocardial injection of naked plasmid DNA encoding human atrial natriuretic peptide 兔心肌內注射人心房鈉尿肽基因裸質(zhì)粒的生物活性和安全性
- Expressions of Liposome-mediated and Naked Plasmid DNA Encoding Human Atrial Natriuretic Peptide in Cultured Rat Ventricular Myocytes 人心鈉素基因裸質(zhì)粒與脂質(zhì)體介導轉染在培養鼠心肌細胞中表達的研究
- Intramuscular injection of naked plasmid DNA encoding human preproinsulin gene in streptozotocin-diabetes mice results in a significant reduction of blood glucose level 人前胰島素原基因裸質(zhì)粒DNA肌肉注射可顯著(zhù)降低鏈脲佐菌素誘發(fā)糖尿病小鼠的血糖水平
- Plasmid DNA has important function of gene regulation, direct injection of naked or Lipid - coated plasmid DNA often requires considerable amount of plasmid DNA. 質(zhì)粒DNA有重要的基因調節性能，直接注射裸露或用脂質(zhì)體包埋的質(zhì)粒DNA常需要大量的質(zhì)粒DNA。
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四頭肌可檢測到外,其余組織中均為陰性;
- Objective To develop a procedure for large-scale purification of plasmid DNA. 摘要目的建立適合大規模純化質(zhì)粒DNA的工藝。
- The serum from anemic rats treated with naked plasmid expressing human EPO showed obvious neutralizing activity to inhibit the proliferation of BEF-2 cells. 在大鼠模型中,表達人EPO基因裸質(zhì)粒組的血清有明顯的中和活性,可抑制BEF-2細胞的增殖。
- P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III. 擴增p 16 promoter一luc一pGLZ一Basic報告基因表達質(zhì)粒并提取其DNA。
- This arti cle reviews the structure of plasmid DNA, especially the structure of its Assist ant units and effects. 本文闡述了基因免疫中質(zhì)粒DNA中的結構組成特別是其佐劑單位及其作用。
- Conclusion Naked plasmid with mutant human proinsulin gene is successfully transfected into diabetic mice, which show the singificant short-term effect of decreasing blood glucose. 結論含有人胰島素原突變基因的裸質(zhì)粒成功轉染糖尿病小鼠,并有顯著(zhù)的短期降血糖效應。
- Plasmid DNA and calf-DNA are irradiate d by laser with the samestrengty and different irradiation period. 激光作用質(zhì)粒DNA和小牛胸腺DNA產(chǎn)生損傷效應，導致DNA結構變化。
- The traditional alkaline method to isolate plasmid DNA takes time and the operation system requires strict control. 實(shí)驗室中常用的質(zhì)粒提取方法是堿裂解法。
- Switch on the vacuum to draw the solution through the column (The plasmid DNA binds to the column material). 開(kāi)啟真空裝置使液體通過(guò)管柱(質(zhì)體DNA會(huì )與管柱物質(zhì)結合)。
- Ion exchange HPLC is an alternative that yields milligram quantities of purified plasmid DNA within an hour. 離子交換HPLC作為替代方法可以在一小時(shí)內生產(chǎn)毫克量的純化的質(zhì)粒DNA。
- Routine alkaline lysis miniprep of plasmid DNA was modified,and LiCl was specially used. 對堿裂質(zhì)粒小量法進(jìn)行了改進(jìn)，并且在提取過(guò)程中增加了LiCl處理。
- TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer. TNA可促進(jìn)脂質(zhì)體介導質(zhì)粒DNA轉染結直腸癌細胞 ,將營(yíng)養支持與基因治療相結合 ,有望提高對腫瘤患者支持治療效果。
- Methods Plasmid DNA was extracted by the procedure of Kado and Liu,and Pgm was detected by incubating isolates on Congo red media at28oC for7days. 方法質(zhì)粒運用Kado改良法進(jìn)行提取; Pgm利用剛果紅培養基置28℃培養7天觀(guān)察。
- A variety of approaches have been taken to augment and modulate the immunogenicity and efficacy of plasmid DNA vaccines. 核酸疫苗雖然在小動(dòng)物實(shí)驗中取得成功,但對大動(dòng)物、靈長(cháng)類(lèi)動(dòng)物和人類(lèi)的免疫原性卻很低。
- Assuming that the plasmid DNA is endotoxin-free, mention how you think "danger" is signaled by DNA of bacterial or plasmid origin (5 points). 假設這個(gè)質(zhì)體DNA不含內毒素，提出理由說(shuō)明你認為"危險"訊息是源自細菌的DNA或是質(zhì)體（5分）。
- The damage effect of plasmid DNA and mutation induced of lacZ gene were analysesed by low power HeNe laser radiation invitro plasmid DNA. 利用低功率HeNe激光輻照離體質(zhì)粒DNA，分析了質(zhì)粒DNA的損傷效應和lacZ基因的突變。
