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- Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid. 方法載體的構建采用限制性?xún)惹忻该盖小?DNA連接酶連接等方法。
- In GENETIC ENGINEERING, the joining of DNA fragments by the action of the enzyme DNA ligase. 在基因工程中，在DNA連接酶的作用下連接DNA片段的過(guò)程。
- With T4 DNA ligase link the GFP gene to pET-MP, the constructed plasmid named after pET-MP-GFP. 用T4連接酶將gfp基因連接到pET-MP上，得到的質(zhì)粒命名為pET-MP-GFP。
- Once a plasmid is snipped open, a foreign piece of DNA, cut by the same enzyme scissors, can be taped, end to end, into the plasmid using another enzyme, DNA ligase. 一旦原生質(zhì)被分開(kāi)后，用同樣限制酶剪開(kāi)的外來(lái)DNA片段和它的被切斷的端口被粘到一起，形成了新的原生質(zhì)。連接時(shí)使用的是另一種酶--DNA連接酶。
- DNA ligase An enzyme, used in DNA REPLICATION and the in vitro synthesis of DNA, that joins together segments of newly synthesized polynucleotide chains using energy from ATP. DNA連接酶：是一種在DNA復制或DNA晶體合成中起作用的酶，它能夠將新合成的DNA片段連接在一起，反應中需要ATP提供能量。
- DNA ligase An enzyme, used in DNA REPLICATION and the in vitro synthesis of DNA, that joins together segments of newly synthesized polynucleotide chains using energy from ATP. See replication. DNA連接酶：是一種在DNA復制或DNA晶體合成中起作用的酶，它能夠將新合成的DNA片段連接在一起，反應中需要ATP提供能量。參見(jiàn)replication.
- Recent Advances in DNA Ligase Isozymes DNA連接酶同工酶的研究進(jìn)展
- Keywords molecular beacon;DNA ligase;polymerase;biomolecule;detection; 分子信標;連接酶;聚合酶;生物分子;檢測;
- Keywords Plasma DNA;Point mutation;DNA ligase;Real-time PCR;Capillary electrophoresis;Gene array; 血漿DNA;點(diǎn)突變;DNA連接酶;熒光定量PCR;毛細管電泳;基因芯片;
- Comparison between the T_4DNA ligase activity determined by DNA ligase self-adenylation and ATP-~(32)PPi exchange reaction T_4DNA連接酶活性的自身腺苷化和交換法測定的比較
- Biochemical and Enzymatic Characterization of a Thermostable DNA Ligase Encoded by Thermophilic Acidophilic Archaebacterium Strain JP2 一種嗜熱耐酸古細菌JP2菌株編碼的熱穩定DNA連接酶的生物化學(xué)及酶學(xué)特性研究(英文)
- Keywords DNA Ligase;Alkaline phosphatase;biocatalytic deposition;point mutation;genotype;electrochemical immunoassay;catalytic hydrogen electrochemical determination; 關(guān)鍵詞DNA連接酶;堿性磷酸酶;生物催化沉積;點(diǎn)突變;基因分型;電化學(xué)免疫試驗;催化氫波電化學(xué)檢測;
- El triplete de Liga, Copa y Champions sigue a tiro. 巴薩進(jìn)入了歐冠決賽，繼續著(zhù)他們三冠王的夢(mèng)想。
- Primera Liga Zhanmu open, beautiful Mata continue. 與一戰，馬塔83分鐘的絕殺為瓦倫西亞拿回3分。
- Liga_5_Star Spletna igra Liga 5 Star. 東莞豪華大酒店四星級園林式酒店。
- Keywords Single Nucleotide Polymorphisms （SNPs）;DNA ligase;Colorimetric;Molecular beacon;Aptamer beacon;Bio-species immobilization;Biosensor;Immunoassay; 單核苷多態(tài)性;DNA連接酶;比色法;分子信標;分子信標識體;生物組分固定化;生物傳感器;免疫分析;
- DNA fingerprinting can help the police to find the criminals. DNA指紋術(shù)有助於警察發(fā)現罪犯。
- CL was a potent inhibitor of Ca 2+ ,Mg 2+ ATPase. 馬桑內酯是Ca2＋，Mg2＋?ATP酶的強力抑制劑。
- Usually, Clr is binding to C1INH (Cl inhibitor). 補體組分C1r含有兩個(gè)CUB結構域，一個(gè)EGF結構域和一個(gè)絲氨酸蛋白酶結構域，是一種絲氨酸蛋白酶原。
- Mouldproof wall paper, join mould inhibitor. 防霉墻紙，加入防霉劑。
