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- Comparison between the T_4DNA ligase activity determined by DNA ligase self-adenylation and ATP-~(32)PPi exchange reaction T_4DNA連接酶活性的自身腺苷化和交換法測定的比較
- Active site motif 活性部位基序
- Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid. 方法載體的構建采用限制性?xún)惹忻该盖小?DNA連接酶連接等方法。
- In GENETIC ENGINEERING, the joining of DNA fragments by the action of the enzyme DNA ligase. 在基因工程中,在DNA連接酶的作用下連接DNA片段的過(guò)程。
- With T4 DNA ligase link the GFP gene to pET-MP, the constructed plasmid named after pET-MP-GFP. 用T4連接酶將gfp基因連接到pET-MP上,得到的質(zhì)粒命名為pET-MP-GFP。
- Once a plasmid is snipped open, a foreign piece of DNA, cut by the same enzyme scissors, can be taped, end to end, into the plasmid using another enzyme, DNA ligase. 一旦原生質(zhì)被分開(kāi)后,用同樣限制酶剪開(kāi)的外來(lái)DNA片段和它的被切斷的端口被粘到一起,形成了新的原生質(zhì)。連接時(shí)使用的是另一種酶--DNA連接酶。
- DNA ligase An enzyme, used in DNA REPLICATION and the in vitro synthesis of DNA, that joins together segments of newly synthesized polynucleotide chains using energy from ATP. DNA連接酶:是一種在DNA復制或DNA晶體合成中起作用的酶,它能夠將新合成的DNA片段連接在一起,反應中需要ATP提供能量。
- Allosteric enzymes possess an allosteric site in addition to their ACTIVE SITE. 別構酶控制著(zhù)別構部位和它的活性部位。
- They contain nonheme iron in association with sulfur at the active site. 它們含有非血紅素鐵,在活性位點(diǎn)與硫協(xié)同作用。
- You should check site statistics at least monthly if you have an active site. 如果你有一個(gè)活躍的站點(diǎn),你至少應該每月都查看一下站點(diǎn)的統計表。
- DNA ligase An enzyme, used in DNA REPLICATION and the in vitro synthesis of DNA, that joins together segments of newly synthesized polynucleotide chains using energy from ATP. See replication. DNA連接酶:是一種在DNA復制或DNA晶體合成中起作用的酶,它能夠將新合成的DNA片段連接在一起,反應中需要ATP提供能量。參見(jiàn)replication.
- Unlike organic inhibitors, POMs do not bind to the active site of the enzyme. 不同于有機的抑制劑, 多金屬氧酸鹽摻雜聚苯胺電子聚合物 不是抑制活性部位的酶。
- Most active site residues are polar, including some charged amino acids. 大多數活化物殘留物都有極性,包括一些帶電氨基酸。
- Recent Advances in DNA Ligase Isozymes DNA連接酶同工酶的研究進(jìn)展
- For instance, they might construct a compound that inhibits the active site of an enzyme crucial to viral reproduction. 舉例來(lái)說(shuō),他們可以設計某個(gè)化合物,作用在病毒復制時(shí)不可或缺的酵素活性區,而加以抑制。
- When you create a site collection, the site is added to the database and is logged it as an active site. 如果創(chuàng )建網(wǎng)站集,會(huì )將網(wǎng)站添加到數據庫中并將其記錄為活動(dòng)網(wǎng)站。
- High temperature active site was unfavourable to the hydrogenolysis, its hydrogenolysis behavior showed little effect on DHC. 高溫活性中心也有一定的氫解活性,但對脫氫環(huán)化反應的抑制作用較小。
- The reaction is stoichiometric and there is no regeneration of the active site and no turnover of AGT. 過(guò)度表達AGT的轉基因鼠可免患烷基致癌劑誘導的腫瘤,無(wú)AGT活性的小鼠對甲基亞硝基脈的致癌效應尤其敏感。
- The copper complex moiety in SOD has been well documented as the active site for the catalytic dismutation of O_2~- . 文獻已經(jīng)證實(shí),SOD中的銅離子是催化歧化超氧陰離子的活性中心。
- On the basis of the modeling, the components and structure of active site in CYP2f1 were analyzed. 在此基礎上;分析了活性位點(diǎn)的組成和結構;并進(jìn)行了與小分子(維甲酸)的對接研究.