DNA content assay and cell apoptosis detection kit were used to assessed the apoptosis by flow cytometry. Semi-quantitative reverse transcription-PCR was performed to detect mRNA expression level of bcl-2, IL-6, IL-6R and Mcl-1 in NCI-H446 cell line.
英
美
- 方法：通過(guò)MTT實(shí)驗、細胞克隆形成實(shí)驗觀(guān)察各組反義藥物對細胞增殖的影響，應用細胞凋亡線(xiàn)粒體流式檢測和DNA倍體分析觀(guān)察各組藥物對細胞凋亡的影響，建立半定量RT-PCR，檢測各組藥物作用后的NCI-H446細胞株bcl-2、IL-6、IL-6R、Mcl-1等相關(guān)基因mRNA表達水平的變化。
