Comparision of their maps, 40 different spots were digested in gel with TPCK-trypsin and measured with MALDI TOF MS, and 20 protein were identified, which may help to search mechanism of the development of lateral root.
英
美
- 分析其不同部位的雙向電泳圖譜的蛋白質(zhì)表達差異,應用胰蛋白酶膠內酶解及基質(zhì)輔助激光解吸/電離飛行時(shí)間質(zhì)譜(MALDI-TOFMS)的肽質(zhì)指紋技術(shù)對40個(gè)差異表達蛋白質(zhì)進(jìn)行鑒定,最終鑒定出20個(gè)蛋白,從而為側根發(fā)生機理的研究提供科學(xué)依據。