Co-cultures of mouse oocytes with pBR322 -HBV DNA plasmids were performed and the entering of HBV DNA into the oocytes and its integrating in host chromosomes were demonstrated using PCR, dot and Southern hybridizations and FISH techniques.
英
美
- 將小鼠卵母細胞與pBR322-HBV DNA質(zhì)粒共培養,用斑點(diǎn)雜交、PCR、Southern雜交與FISH技術(shù)證實(shí)HBV DNA進(jìn)入卵母細胞并在卵母細胞染色體上整合。