Cells were then transfected with AAV-hBMP4 and AAV-EGFP respectively. The ossification of cells was evaluated by morphological observation,alkali phosphatase stain,Von Kossa stain and alkali phosphatase assay.
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- AAV-hBMP4與對照病毒AAV-EGFP分別轉染細胞,觀(guān)察細胞形態(tài),行堿性磷酸酶(ALP)染色、Von Kossa染色及ALP含量測定,觀(guān)察成骨活性。
