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- The relative binding affinities (RBA) of four steroid hormonal drugs for estrogen receptors of uterine cytosol were detemined by competitive binding assay and shown to be in the following order: ethinyl ostradiol>estradiol>anordrin>danazol. 根據[~3H]E_2和E_R具有高度專(zhuān)一性及親和力的特性,應用競爭性試驗比較了炔雌醇(EE)、雌二醇(E_2)、雙炔失碳酯(Anordrin)、丹那唑(D)與兔子宮胞液雌激素受體的相對結合親和力;
- protein competitive binding assay 蛋白競爭結合分析
- Rat uteri estrogen receptor (ER) competitive binding assay; 大鼠子宮雌激素受體(ER)競爭結合試驗;
- Microbial receptor competitive binding assay of basic macrolide antibiotics 堿性大環(huán)內酯類(lèi)抗生素的微生物受體競爭結合分析
- competitive binding assay 競爭性結合測定
- Competitive protein binding assay indicates the level of cAMP is improved when CDCA is given by oral administration. 通過(guò)競爭性蛋白質(zhì)結合分析法測得,鵝去氧膽酸經(jīng)灌胃給藥后能顯著(zhù)提高大白鼠氣管組織中cAMP的含量。
- Competitive inhibition assay, saturation binding assay and scatchard analysis testify the expression of LH/hCG receptor. 競爭結合實(shí)驗、受體結合的特異性實(shí)驗、飽和結合實(shí)驗證明3AO細胞上存在特異性的Ll壬七CG受體。
- Our conclusion is that by competitive inhibition assay, saturation binding assay and Scatchard analysis, LH/hCG receptors with high affinity are present on 3AO cells. 結論: 競爭結合實(shí)驗、特異性結合實(shí)驗、飽和結合實(shí)驗表明 3AO細胞膜上存在高親和力的LH/hCG受體。
- Binding assay shown that Vp28 can not binding membrane protein of shrimp gill. 純化的VP28與對蝦鰓細胞膜的結合實(shí)驗表明,VP28不能與對蝦鰓細胞膜結合,即純化的VP28不具有結合活性。
- The dopaminergic receptors were measured with the radio-ligand binding assay. 采用同位素標記法測定受體變化;
- KI quenching and competitive binding between Mn-Schiff -base and ethidium bromide for DNA were studied. 其紫外可見(jiàn)光譜亦有明顯的減色效應和微紫移。
- Therefore, ATF gene transfer results in the suppression of spontaneous metastasis through competitive binding of exogenous ATF to urokinase receptor with urokinase. 上述結果揭示ATF基因轉染后的腫瘤細胞通過(guò)ATF基因的表達與尿激酶競爭其受體的結合可在一定程度上抑制腫瘤細胞的惡性表型。
- SSR was detected by radioligand binding assay(RBA) and ER, PR by LsAB method in these groups. 采用RBA法測定各組織的SSR表達 ,采用免疫LsAB法測定ER、PR表達。
- The effects of l-CSL on recombinant D_1R were investigated by [3~H]SCH23390 binding assay and cAMP assay. [~3H]SCH23390受體結合和cAMP測定實(shí)驗檢測左旋氯代斯闊任對重組D_1受體的激動(dòng)作用。
- Method H?3?cAMP was used in protein binding assay to determine the level of cAMP in bovine BTC. 方法運用蛋白競爭結合法用氚(H3)標記的環(huán)磷酸腺苷(adenosine3,5?cyclicmonphos?phate,cAMP)測定牛眼小梁細胞內cAMP的含量。
- Methods: The number and affinity of IR on hepatocyte membrane of ASR were determined with radioligand binding assay. 采用放射配體結合分析方法檢測肝細胞膜胰島素受體的數目與親和力。
- Tamoxifen competitively binds estrogen receptors. 三苯氧胺與雌激素受體結合力。
- Methods Radioligand binding assay was used to determine the contents of GR and its affinity (Kd). 方法應用胃泌素受體的放射配基結合分析法,測定34例胃癌組織胃泌素受體的含量及其親和力。
- HEK293 cells transcripted with EDG 1, a SPP receptor, were cultured and harvested, and incubated with 32 P labeled or non labeled SPP. The SPP level was determined based on competitive binding of SPP or 32 P SPP to HEK cells. 通過(guò)培養和收集轉染SPP受體 EDG 1的HEK2 93細胞 ;與標記及非標記SPP共孵育 ;利用它們與HEK2 93細胞的競爭性結合 ;測定細胞、血清和組織中SPP含量 .
- Methods: Wrap restraint stress induced defecation rats, the guanylin contents in colon and plasma were measured by radioligand binding assay. 方法:建立束縛應激誘發(fā)排便異常大鼠動(dòng)物模型,應用放射配基法測定結腸組織及血漿中鳥(niǎo)苷素含量。