By the inactivated protoplast fusion method carrided out, fusants was selected through the means by TTC,and the fusant RHZ-7 was obtained which can produce 9.27% (v/v) alcohol and 0.721g/L ester.

 
  • 采用紫外線(xiàn)滅活MT-14原生質(zhì)體后與呼吸缺陷型突變株HX-9進(jìn)行原生質(zhì)體融合;在再生培養基平板上挑選TTC反應呈紅色的融合子;并經(jīng)過(guò)分級篩選;得到一株產(chǎn)酒精達9.;27%25(v/v)、產(chǎn)酯0
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