By the BP recombination reaction,the PCR product containing attB was transfered to an attP-containing donor vector to create an entry clone. Finally,DREB1A gene was shutted into pH2GW7 vector by LR recombination reaction.
英
美
- 通過(guò)BP反應將包含有attB接頭的PCR產(chǎn)物克隆到含有attP的donor載體上以產(chǎn)生Entry克隆,通過(guò)LR反應將已經(jīng)重組入Entry載體的DREB1A基因再克隆到pH2GW7雙元載體。
