Based on the analysis results, three partition of VP1 gene of CAV were cloned and inserted into the bacterial plasmids pGEX-4T-1. The recombinant plasmids containing VP1 partiton gene of CAV were identified by restriction enzyme analysis and PCR method.

 
  • 然后將VP1基因分段克隆至原核表達載體pGEX-4T-1,經(jīng)酶切及PCR鑒定,證明成功構建了重組表達載體pGEX-A、pGEX-B、pGEX-C。
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