At first, the gpd gene was cloned. In order to studying the relationship between gpd gene and V. volvacea cold shock response, the expression pattern of gpd was performed in low temperature.
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- 首先，本實(shí)驗通過(guò)RACE的方法克隆了草菇的gpd基因，從轉錄水平上探討了草菇gpd基因在低溫條件下的表達量情況，以期了解gpd基因與草菇低溫應答的關(guān)系，建立了以gpd為內標基因檢測草菇冷誘導相關(guān)基因在低溫條件下轉錄水平的半定量RT-PCR方法。
