Apo-HDL was applied to Sephadex G-150 and DEAE-cellulose column for purification of Apo A-T. The purified Apo A-I was used to immunize New Zealand rabbits and the specific antiserum to human Apo A-I (effioiency 1:32) was collected.
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- 經(jīng)Sephadex G-150層析及DEAE-纖維素(DE-52)離子交換得純化載脂蛋白A-I(Apo A-I),免疫新西蘭兔,獲得效價(jià)為1:32的特異性抗人Apo A-I抗血清。