After treatment with PBS containing 5% normal serum for 20 mins, the sections were incubated with monoclonal Tiam -1 antibody at a dilution of 1:100 for 2 hours in a moist chamber.

 
  • 滴加1:50封閉用正常血清室溫孵育20分鐘,PBS稀釋一抗Tiam-1(1:100)室溫孵育2小時(shí)或4℃過(guò)夜;
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