After the protein was stained with a mixture of coomassie blue R-250 in 10% acetic acid-30% mcthanol, SDS-PAGE analysis yield two bands when the sample was reduced with 2-ME, IgY dissociated into a heavy chain and a light chain of 70KD and 40KD.

 
  • 凝膠經(jīng)考馬斯亮藍染色后出現兩條蛋白帶,它們的分子量分別為70KD和40KD,這兩條蛋白帶為免疫球蛋白經(jīng)SDS-PAGE變性電泳后裂解的兩個(gè)亞基。
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