After the fetal umbilical vein endothelial cells are collected,inoculate them on 96 pores plate. Then add PG cells marked with 5,6-CFDA,and add SQ of different concentration to SQ groups.

 
  • 胎兒臍靜脈內皮細胞收集后,接種在96孔板上,然后加入已標記5,6-CFDA的PG細胞,實(shí)驗組分別加入不同濃度的SQ,CO2培養箱中培養60min,再加入CD34-PE與粘附的細胞孵育30min,在熒光顯微鏡下檢測PG與內皮細胞的粘附率。
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