After mice were immunized with BoNTB/Hc, the spleens were harvested ,and single-chain Fv (scFv) and Fab phage antibody libraries were constructed from the immunoglobulin heavy and light chain variable region genes.

 
  • 用重組表達并純化的BoNTB/Hc免疫小鼠;從其脾淋巴細胞中提取全套抗體可變區基因;分別構建scFv和Fab段噬菌體抗體文庫;庫容分別為6.;72x10~7cfu和5
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