A transfering vector was constructed by inserted NDV F gene and IBDV VPO gene, under the control of two synthetic promoters ATI P7. 5X20 which were conjugated at opposite direction inserted within the thymidine kinase (TK) gene of FPV 282E4 strain.
英
美
- 將NDV F基因和IBDV VPO基因分別置于人工合成的復合啟動(dòng)子ATI·p7.；5×20的下游，將兩個(gè)表達單元以復合啟動(dòng)子反向方式連接，插入TK基因中，得到兩個(gè)復合啟動(dòng)子分別調控兩個(gè)不同基因的重組轉移質(zhì)粒pVPO-2ATI·p7
