A quantitative competitive PCR(qcPCR) assay was used to detect CD80-CD86 mRNA levels in porcine alveolar macrophages(PAMs) during PCV2 infection through construction of their respective competitive depletion clones.
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美
- 通過(guò)構建豬共刺激分子CD80和CD86的缺失cDNA競爭分子,用競爭PCR技術(shù)定量檢測了豬圓環(huán)病毒2型(PCV2)感染后豬肺泡巨噬細胞(PAM)中CD80和CD86的mRNA水平,分析了PCV2感染對豬共刺激分子CD80和CD86的mRNA表達的影響。
