A pair of specific primer was designed for amplification of cry2Ab gene. The amplified products of cry2Ab gene was directly inserted into pUCm-T vector and transformed into E.

 
  • 根據已知的cry2Ab基因ORF序列,設計合成了一對特異性引物,PCR擴增出cry2Ab基因全長(cháng),并直接與pUCm-T載體相連。
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