A mouse combinatorial Fab library was constructed by inserting both light chain gene and Fd gene fragments into phagemid vector pComb3, and followed by the help phage VCSM 13 infection. The recombination rates of light chains and Fd fragments were 80%.
英
美
- 通過(guò)PCR反應和雙酶切反應鑒定抗體庫的重組率,輕鏈、重鏈Fd段基因的重組率均為80%25。 抗體Fab段基因庫經(jīng)過(guò)輔助噬菌體VCSM13超感染,成功構建了鼠源性噬菌體Fab抗體庫。