The reduction was conducted in Tris-HCl buffer, and the concentrations of COBE and CHBE and the optical purity of product were determined by capillary GC and HPLC equipped with a chiralcel OB column, respectively. The product was mainly D-(S)-CHBE.
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- 實(shí)驗發(fā)現 ;在水相體系中主要生成 D (S) 型產(chǎn)物 ;COBE的轉化率及CHBE的收率和光學(xué)選擇性都比較高 .