3) Purified recombinant Ad plasmid DNA is digested with PacI to expose its inverted terminal repests( ITR), and then used to transfect AD-293 cells where deleted viral assembly genes are complemented in vivo.

 
  • 3) 用線(xiàn)性化的重組腺病毒轉染293細胞,獲得基因組結構均一的重組腺病毒。
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