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- Methods:PCR,denatur ing gradient gel electrophoresis (DGGE) atd DNA sequencing were used. 方法:采用多聚酶鏈反應(PCR),變性梯度凝膠電泳(DGGE)和DNA測序等方法對上海地區血友病甲基因突變進(jìn)行檢測。
- Denaturing gradient gel electrophoresis(DGGE) is an advanced molecular fingerprinting technique. 變性梯度凝膠電泳(DGGE)是近些年微生物分子生態(tài)學(xué)研究中的熱點(diǎn)技術(shù)之一。
- The results showed that soil samples collected from same area had the high identity in DGGE bands patterns. 結果表明,采自同一地區的土壤樣品,其DGGE圖譜的相似性很高;
- Through physiological and biochemistry experiments and 16SrDNA sequence analysis, the two marine bacteria strains CHS and SY, were further identified as Pseudoalteromonas. 從分離到的217株細菌中,獲得23株堿性蛋白酶酶活高的菌株,對其中兩株海洋細菌CHS和SY菌株進(jìn)行生理生化和16SrDNA序列分析,初步鑒定為交替假單胞菌。
- The DGGE band number fromthe subsoil samples was obviously more thanthat fromthe soil samples around the pond. 底泥樣品的DGGE條帶數明顯多于池塘周邊土壤樣品。
- Results shown that the PCR product of bacterial nucleic acid, which was purified with modified CTAB method, obtained stablethe most bands in DGGE. 結果表明,改良CTAB法抽提獲得細菌核酸的PCR產(chǎn)物在DGGE凝膠上獲得的條帶最多,結果穩定。
- What could you conclude from PCR/DGGE analysis of a simple that yielded one band by PCR and one band by DGGE?One band by PCR and for band by DGGE? 前者說(shuō)明該條帶具有固定的序列及片段大小相同,后者說(shuō)明片段大小雖然相同,但序列還是存在差異。
- Some DGGE strip lack or increase showed that fungus group had changed differently in rhizosphere and non-rhizosphere soil. 真菌數量有所增加,種群發(fā)生了明顯改變,表現為條帶的缺失和增加。
- In this paper, techniques, such as Biomarker, SCSU, DNA renaturation experiment, DGGE, TGGE, ARDRA, T-RFLP, SSCP, PAPD and ERIC-PCR, commonly used in this field were introduced. 本文介紹了生物標志物法、SCSU、DNA復性分析、DGGE、TGGE、ARDRA、T-RFLP、SSCP、PAPD和ERIC-PCR 圖譜分析等方法在土壤微生物群落結構研究中的應用。
- Methods Denaturant gradient gel electrophoresis (DGGE) was used to scan all DNA fragments of 3 genes mentioned above in AD group (15 cases) and elderly control group (241 cases). 方法 應用DNA變性梯度凝膠電泳方法對15例AD和241例老年對照組的上述3個(gè)基因的DNA片段進(jìn)行掃描分析。
- Effects of different cropping system on diversity of soil bacterial community were studied by using denatured gradient gel electrophoresis (DGGE) analysis. 摘要 采用變性梯度凝膠電泳(DGGE )技術(shù),研究了不同種植模式對土壤細菌群落多樣性的影響。
- Results showed that the numbers and strength of the bands from DGGE profiles varied with the different cropping system, especially the distinguished bands were different. 結果表明,不同種植模式下DGGE圖譜條帶的數量及亮度有較大區別,且有幾條特征性條帶發(fā)生了明顯的變化。
- This article reviewed the principle, merits and limitations of DGGE technique and also reviewed the latest research in the oral cavity with this novel technique. 下面就DGGE的原理、在口腔微生物研究中的應用、優(yōu)缺點(diǎn)和應用前景作一綜述。
- A method based on PCR was developed to amplify short tandem repeat(STR) in combination with denaturing gradient gel electrophoresis (DGGE) and silver dyeing. 應用聚合酶鏈反應擴增STR,經(jīng)變性凝膠電泳,再用銀染顯色方法,分析苯丙氨酸羥化酶(PAH)基因內含子3中TCTA重復序列的多態(tài)性。
- In the paper,the principle of DGGE and its application in food microbiology were reviewed.The application of PCR-DGGE in fermented food research was illustrated outstandingly. 介紹了DGGE的基本原理及其在食品微生物學(xué)研究中的應用,著(zhù)重闡述了PCR-DGGE在發(fā)酵食品研究中的應用進(jìn)展。
- After PCR anplification of total community DNA using a specific primer set,why is it usually necessary to eithercloneor run DGGE on the products before sequencing them? 相同的條帶表示分子量相同但可能序列不同,需通過(guò)DGGE檢測證明是一種DNA序列。
- The results of DGGE and analysis of nucleotide sequences of 28S rDNA showed identical patterns and nucleotide sequences in the serotype A and D of C. neoformans var. neoformans, which were distinct from the serotye B and C of C. neoformans var. gattii. 經(jīng)過(guò)DGGE和DNA序列分析,所有新生隱球菌新生變種(A、D型)具有一致的基因帶型和序列,格特變種(B、C型)具有不同于新生變種的獨特一致的帶型和序列;
- Because of its objective, rapid and accurate assessment character, DGGE has extensively been used on assessment of the diversity and changes of bacterial species in the microbial population. 由于DGGE技術(shù)具有可靠性強、重現性高和方便快捷等優(yōu)點(diǎn),被廣泛地應用于微生物群落多樣性和動(dòng)態(tài)性分析。
- denaturing gradient gel electrophoresis (DGGE) 變性梯度凝膠電泳
- denatured gradient gel electrophoresis(DGGE) 變性梯度凝膠電泳(DGGE)