Methods: Human epidermal keratinocytes(cell line HaCaT) were cultured in vitro. After treated with certain concentration of arsenic,the expressions of CDK2,CDK4 and cyclin-E were measured by immunoblot test.
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美
- 方法:以體外培養的角質(zhì)形成細胞株為對象,一定濃度砷劑處理細胞后,用免疫印跡方法研究HaCaT細胞周期蛋白依賴(lài)激酶2(CDK2)、周期蛋白依賴(lài)激酶4(CDK4)和細胞周期蛋白E(Cyclin-E)的表達情況及砷劑對其表達的影響。
