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- 地高辛標記引物酶顯色法檢測HBV基因多態(tài)性及應用Application of enzyme coloration with digoxin-labeled primers for detection of HBV gene polymorphism
- 方法 應用聚合酶鏈反應（PCR）方法，采用熒光標記引物和377型DNA序列自動(dòng)分析儀，分析21例 GBM 9號染色體上 20個(gè)微衛星多態(tài)性標記的LOH。Methods 20 loci on chromosome 9 were examined to detect loss of heterozygosity(LOH) in 21 cases of glioblastoma by PCR based microsatellite polymorphism analy-ses, in which fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied.
- 熒光標記引物復合擴增短串聯(lián)重復序列技術(shù)用于異基因造血干細胞移植植活測定A study on technique of multiplex polymerase chain reaction with fluorescence labeling primers for short tandem repeats to document engraftment following allogeneic stem cell transplantation and its application
- 任意引物PCRarbitrarily primed PCR
- 標記mark
- 二引物PCRTP-PCR
- 雙引物PCRDouble - primer PCR
- 方法 應用四色熒光標記引物復合擴增技術(shù) ,對鄂倫春族 10 1名無(wú)關(guān)個(gè)體的血樣 15個(gè)STR基因座進(jìn)行分型研究 ,計算各基因座的相關(guān)群體遺傳學(xué)參數。Method DNA samples from 101 unrelated individuals in Chinese Olunchun population were screened by Power Plex 16 System and ABI 3100 Genetic Analyzer.
- 引物primer
- 隨機引物PCRarbitrarily primed PCR(APPCR)
- 組合引物PCR法grouping primers PCR
- 半隨機引物PCRSemi-random primer PCR
- 方法應用聚合酶鏈反應(PCR)方法,通過(guò)熒光標記引物和377型DNA序列自動(dòng)分析儀,對20例原發(fā)性膠質(zhì)母細胞瘤患者7號染色體上的22個(gè)微衛星多態(tài)性標記進(jìn)行雜合性丟失(lossofheterozygosity,LOH)分析。Methods All of the 22 microsatellite polymorphism markers on chromosome 7 in 20 cases with GBM were detected by polymerase chain reaction (PCR) technique with fluorescence-labeled primers and Perkin Elmer 377 DNA sequencer and the loss of heterozygosity (LOH) in them was analyzed.
- 引物原位標記primed in situlabelling; RINS
- 型特異性引物PCRType-specific primer PCR
- 熒光標記引物fluorescent dye primer; fluorescently-labeled primer
- 隨機引物法標記Random Prime Labeling of DNA
- RNA任意引物PCRRNA arbitrarily primed PCR
- 隨機引物標記法random primering
- 因而，設計PCR引物時(shí)宜選擇Takahashi等分離株為標準。In China these viruses share high homology with Takahaski’s strains which might be good standards for primer designation.
