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- 方法:改良CTAB法。Methods: The improved CTAB DNA extraction method.
- 采用改良CTAB法提取河南 17個(gè)桂花品種的基因組DNA ,利用RAPD技術(shù)對其進(jìn)行鑒定和分類(lèi)研究。Applying an improved CTAB method to extract DNA from leaves of Osmanthus fragrans, studied the classification and identification of 17 Osmanthus fragrans cultivars in Henan province by the technique of random amplified polymorphic DNA(RAPD).
- 改良CTAB法提取DNA質(zhì)量好于改良SDS法,改良方法尤其改良CTAB法是一種適合小麥基因組DNA提取的方法。The improved CTAB extraction method which was designed by our research group is suitable to extract the high quality DNA from wheat. Facile operation can save time and be economical.
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- 無(wú)法unable
- 用改良CTAB法提取的總DNA的OD260/OD280在1.6-2.0之間,電泳條帶清晰,其含量和純度完全滿(mǎn)足RAPD分析的要求。The OD260/OD280 of total DNA was between 1.6 to 2.0 with the improved method of CTAB,the banding patterns was clear,and the content and pureness of the total DNA can meet the reaction for RAPD.
- 改良improve
- 用法usage
- 說(shuō)法statement
- 改進(jìn)的CTAB法DNA得率高于改進(jìn)的SDS法;DNA yield rate of modified CTAB method was higher than modified SDS method.
- 改良的improved
- 輸入法input method
- 改良CTABCRAB
- 除法division
- 分析法analytical method
- PCR方法檢測轉基因食品時(shí),應用改良CTAB法制備DNA模板。DNA extract by using modified CTAB methods was suitable for PCR-based detection of genetically modified organisms in foods and animals feed.
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