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- 1、我們通過(guò)定向克隆的方法，成功地構建了含EWS-FLI-1特異結合序列和白喉毒素A鏈基因的真核表達載體。1. The expression vector pS2-DTA contains the EWS-FLI-1 binding sequence and the DTA gene sequence.
- 釋放質(zhì)粒后，進(jìn)行酶切鑒定，LycB陽(yáng)性克隆cDNA插入片段的大小為2.1kb左右，IPI陽(yáng)性克降cDNA插入片段的大小約為1.5kb。The recombinants with the cDNA insert fragment were released from the positive clones and were digested with EcoR I , The cDNA fragment were 2.1kb and 1.5kb in lycB and IPI positive clones respectively.
- 鼻咽上皮組織定向cDNA文庫的快速構建Rapid construction of directional cDNA library from human nasopharynx
- 釋放質(zhì)粒后，進(jìn)行酶切鑒定，該陽(yáng)性克隆cDNA插入片段的大小為2kb左右。Thin recombinant with the cDNA ~crt fragment was released frc the positive clone and was di ?z; tcJ with EcoRI; the eDNA fra, ent was 2kb in length.
- 文庫library
- 從基因組DNA中PCR擴增一段含Runt結合順序的MDK基因啟動(dòng)子/增強子序列(MDKp)，定向克隆到pGL3-Basic質(zhì)粒，構建MDKp-pGL3-Basic Luciferase報告基因表達載體；PCR. The MDK promoter/enhancer (MDKp) sequence with runt domain binding sites was amplified from the human genome DNA and directionally cloned into pGL3-Basic Luciferase reporter vectors to construct the MDKp-pGL3-B expression plasmids.
- 克隆clone
- 副溶血弧菌感染12h和24h雜色鮑肝臟全長(cháng)cDNA文庫的構建Construction of liver full-length cDNA libraries of abalone Haliotis diversicolor infected with Vibrio parahaemolyticus for 12 h and 24 h
- AD-cDNA文庫AD-cDNA library
- 方法通過(guò)PCR方法擴增獲得博爾納病病毒p40基因的完整序列,將此片段定向克隆到pEGFP-N1載體多克隆位點(diǎn)區,篩選重組陽(yáng)性菌株,提取重組質(zhì)粒,利用PCR方法和核酸序列測定驗證重組質(zhì)粒構建的正確性。Methods The target DNA fragments of BDV p40 gene was obtained by PCR amplification and inserted into pEGFP-N1 reporter vector. The constructed recombinant plasmid was confirmed by PCR and DNA sequencing.
- 褐飛虱誘導的水稻cDNA文庫的構建及鋅指蛋白基因的分離Construction of Rice cDNA Library Induced by Brown Planthopper and Isolation of Dof Zinc Finger Protein
- 丁酸鈉誘導MCF-7細胞凋亡反義cDNA文庫的構建和初步鑒定Construction of an anti-sense cDNA library of MCF-7 cells during apoptosis induced by sodium butyrate and preliminary identification
- 二烯丙基二硫誘導人胃癌細胞差異表達基因cDNA文庫的建立Construction of a subtracted cDNA library of differentially expressed genes in human gastric carcinoma induced by diallyl disulfide
- 強優(yōu)勢玉米雜交種豫玉22雌穗均一化cDNA文庫的構建與鑒定Construction and Identification of a Normalized Maize Ear cDNA Library of Hybrid Y22
- 全長(cháng)cDNA文庫Full-length cDNA library
- 消減cDNA文庫subtractive cDNA library
- 皺紋盤(pán)鮑(Haliotis discushannai Ino)胚胎RNA分離和cDNA文庫構建ISOLATION OF RNA AND CONSTRUCTION OF FULL-LENGTH CDNA LIBRARY OF EMBRYO HALIOTIS DISCUS HANNAI INO
- cDNA文庫基礎上運用熱啟動(dòng)聚合酶鏈反應末端延伸快速分離全長(cháng)cDNA序列Rapid Isolation of Full-Length cDNA Sequence from cDNA Library by Hot-Start Polymerase Chain Reaction
- 黑胸大蠊若蟲(chóng)免疫兔血清對美洲大蠊若蟲(chóng)cDNA表達文庫的篩選及克隆分析Immunoscreening of the Periplaneta americana Nymph cDNA Library with Periplaneta fulignosa Immunized Rabbit Sera
- 褐飛虱取食水稻幼苗cDNA文庫的構建及篩選Construction and screening of a cDNA library from rice seedlings exposed to brown planthopper
