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- 采用Trizol試劑對病毒進(jìn)行了核酸提取,電泳結果發(fā)現有4條核酸條帶,分別為3.0kb、1.3kb、0.8kb、0.75kb,對RNA核酸酶、S1核酸酶敏感,對DNA酶抵抗,為單鏈RNA病毒。The nucleic acid of virus was extracted by using the Trizol reagents and analyzed with 1%25 agarose electrophorosis. It shows the nucleic acid has four bands sized 3.0, 1.3, 0.8 and 0.75 kb respectively, and is sensitive to RNase and S1 nuclerase and resistant to DNase, indicating these bands as single?strand RNA.
- 背景和目的：博爾納病病毒（Borna disease virus, BDV）是一種有套膜的非分節段的單分子負鏈RNA病毒。Background and Objective:Borna disease virus (BDV) is an enveloped, nonsegmented, mononegative RNA virus.
- 雙鏈RNA病毒誘導的牙鲆胚胎細胞差減cDNA文庫的構建Construction of a Subtractive cDNA Library from the Paralichthys olivaceus Embryonic Cells Induced by a Double-Stranded RNA Virus
- 病毒virus
- HIV-1逆轉錄酶(reverse transcriptase RT)在病毒生命周期中起重要作用。 病毒通過(guò)逆轉錄酶將單鏈RNA逆轉錄為雙鏈DNA，隨后病毒DNA迅速整合到宿主DNA中，從而完成轉錄、翻譯等整個(gè)生命周期。The reverse transcriptase (RT) plays a crucial role for the replication of HIV type-1 (HIV-1) in the virus life cycle, and is responsible for the convertion of the single-stranded RNA viral genome into double-stranded DNA that subsequently integrates into the host DNA.
- 雙鏈RNA表達dsRNA expression
- 單鏈RNAsingle-stranded RNA
- 短鏈RNA干擾short interfering RNA
- 雙鏈RNA分解酶dsRNA dependent ribonuclease
- 單鏈線(xiàn)single catenary
- 單鏈噬菌體single stranded phage
- 單鏈DNAsingle-stranded DNA; ssDNA
- 雙鏈RNA激活的蛋白激酶(PKR)的克隆表達及其對丙型肝炎病毒蛋白合成的抑制作用Cloning and Expression of Double-Stranded RNA Activated Protein Kinase(PKR) and Its Effect on Hepatitis C Virus Internal Ribosome Entry Site(IRES) Directed Protein Synthesis
- 單鏈環(huán)single-stranded loop
- 雙鏈RNA依賴(lài)的蛋白激酶(KR)脊椎動(dòng)物中由干擾素介導的抗病毒防御機制的重要組成部分。The double-stranded- RNA-dependent protein kinase( PKR) is an important component in an antiviral defence pathway that is mediated by interferon( IFN) in vertebrates.
- 單鏈泡single-strand bubble
- 單鏈Fabsingle chain Fab
- 單鏈表Single linked list
- 單鏈傳動(dòng)single-chain drive
- 煙草花葉病毒的單份基因組是一個(gè)6395個(gè)核甙的（＋）鏈RNA分子。The monopartite genome of tobacco mosaic virus is a plus-strand RNA molecule of 6395 nucleotides.
