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- . 2.重組表達質(zhì)粒轉染后的AAV-293細胞可檢測到報告基因綠色熒光的表達,根據表達熒光的細胞數得到重組病毒的感染性滴度為106 particles/ml。GFP expression in co-transfected AAV-293 cells was observed and the titer of rAAV is 106particles/ml measured by that cells expressing green fluorescence were counted and compared with the total number of cells.
- 建立了用超臨界制粒的三種方法:快速膨脹超臨界溶液(Rapid Expansive of Supercritical Solutions,即RESS)、超臨界反萃劑(Supercritical Antisolvent,即SAS)和氣體飽和溶液(Particles from Gas-Saturated Solution,即PGSS)方法。Particles for Supercritical Fluid (PSF) includes Rapid Expansive of Supercritical Solutions (RESS), Supercritical Antisolvent (SAS) and Particles for Gas-Saturated Solutions (PGSS) or CO2-Assisted Nebulization Bubble Drying(CAN-BD). PSF overcomes the disadvantages of traditional produced by this approach can be within micro/nanometer. And size with uniform distrubution.
